Vancomycin resistance in Enterococcus gallinarum
نویسندگان
چکیده
منابع مشابه
Analysis of peptidoglycan precursors in vancomycin-resistant Enterococcus gallinarum BM4174.
Vancomycin resistance in enterococci is an increasing clinical problem, and several phenotypes have been identified. We demonstrate here that the resistance mechanism in the constitutively vancomycin-resistant Enterococcus gallinarum BM4174 involves an altered pathway of peptidoglycan synthesis and hydrolysis of the normal precursors in the vancomycin-sensitive pathway. A ligase encoded by the ...
متن کاملMolecular analysis of Tn1546-like elements mediating high-level vancomycin resistance in Enterococcus gallinarum.
OBJECTIVES Four Enterococcus gallinarum isolates, all highly resistant to vancomycin, were studied in order to investigate their relationship and to gain insight into the molecular events responsible for their acquired resistance. METHODS Extensive molecular analysis was performed to compare the four E. gallinarum isolates and their Tn1546-like elements. RESULTS The four strains had very si...
متن کاملvanC cluster of vancomycin-resistant Enterococcus gallinarum BM4174.
Glycopeptide-resistant enterococci of the VanC type synthesize UDP-muramyl-pentapeptide[D-Ser] for cell wall assembly and prevent synthesis of peptidoglycan precursors ending in D-Ala. The vanC cluster of Enterococcus gallinarum BM4174 consists of five genes: vanC-1, vanXY(C), vanT, vanR(C), and vanS(C). Three genes are sufficient for resistance: vanC-1 encodes a ligase that synthesizes the dip...
متن کاملCharacterization of vancomycin resistance in Enterococcus faecium and Enterococcus faecalis.
Vancomycin resistance in Enterococcus faecium 180, a clinical isolate from England, was studied. Resistance to vancomycin was transferable by conjugation to other enterococci. Expression of resistance was inducible and coincided with the appearance of a new membrane protein.
متن کاملRole of the transmembrane domain of the VanT serine racemase in resistance to vancomycin in Enterococcus gallinarum BM4174.
Enterococcus gallinarum BM4175 (a vancomycin-susceptible derivative of BM4174 obtained by insertional inactivation of vanC-1) was transformed with plasmid constructs pCA10 (containing the genes necessary for resistance, vanC-1-XYc-T), pJP1 (with a fragment lacking the DNA encoding the transmembrane region of VanT, -vanC-1-XYc-T((Delta))(2-322)-) and with plasmids containing fragments encoding e...
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ژورنال
عنوان ژورنال: Antimicrobial Agents and Chemotherapy
سال: 1992
ISSN: 0066-4804,1098-6596
DOI: 10.1128/aac.36.7.1392